Basic Info.
After-sales Service
Support
Certification
CE, ISO, IEC, GMP
Stationary Phase
Liquid Solvent
Chromatography Type
Liquid Chromatography
Chromatographic Column Type
Convetional Analytical Column
Chromatographic Column Length
10~30cm
Chromatographic Column Diameter
0.2~0.5mm
Trademark
skyray instrument
Transport Package
Polywood
Specification
568(L)x325(W)x 512mm(H)
Product Description
Product Description
Liquid Chromatograph-single quadrupole mass spectrometer Application usage:Test the main components of known compounds, widely used in pharmaceutical, chemical synthesis, RoHS,REACH,food, environmental protection, industrial analysis and other fields. Working conditions: 1 Working voltage: 220V ± 10%, 50Hz, 10A. ( May use power adapter)2 Working temperature: 20 ºC -30 ºC, 25 ºC± 2 ºC is recommended;3 Humidity: <70% (without dewing water);4 Circumstances: No strong electromagnetic interference; install good ventilation facilities for waste gas and solvent; have the exhaust pipes to the outdoor to blowoff the exhaust gas of the mechanical pump. Product Parameters
1 Single quadrupole mass spectrometer system
1.1 Mass range: 10~2000amu 10~1500(optional) 1.2 Resolution: FWHM of unit mass resolution ≤ 0.6amu 1.3 Mass axial stability: ± 0.2 amu / 12 hrs (under the condition of a constant temperature with ± 2 ºC) 1.4 Mass accuracy: ± 0.2amu 1.5 Sensitivity: ESI +, SIM ion 609.3,10 pg reserpine with direct injection, S / N≥50: 1 (RMS). 1.6 Peak area repeatability: RSD≤4.6% 1.7 Retention time repeatability: RSD≤1.5% 1.8 Scanning speed: Standard mode 1000amu / s, fast scanning mode 10000 amu / s 2 Ion source 2.1 ESI source APCI source(Optional) 2.2 The instrument can ionize in positive or negative ion mode, the polarity switching speed of high-voltage power supply is less than 20ms. (Example: 10KV to -10KV) 2.3 The range of flow rate to ion source: 5uL/min-1000uL/min ESI source, 11uL/min-1000uL/min , the recommended flow rate is 15uL/min); You can configure the lower flow rate according to your needs APCI source 50uL/min-2000uL/min(recommended flow rate is 200uL/min) 2.4 Temperature setting range of ion source: 40-200 ºC 2.5 Capillary voltage of ion source: ± 5000V ; APCI source corona current: 0-10 uA 2.6 Cone voltage range: -120V- +120V, In-source collision acceleration voltage range: 0-120V 3 Scanning function: Full Scan, Selected Ion Monitoring (SIM). 4 MS injection mode 4.1 Syringe pump, manual, auto-sampler three injection methods, The injection pump also has the function of manual flow injection, allowing direct input sample to MS or mass calibration. 4.2 The integrated injection pump can be used for instrument tuning or calibration,Calibration sample can be selected (tuning mix, PPG2000, etc.) 5 Data processing system The software provides automatic control of Auto sampler, LC and MS; the simple full Chinese/English user interface enables high-performance instrument tuning and method optimization, including the optimizations of spray gas pressure, drying gas flow, electrospray voltage, etc;cone voltage, accelerating voltage, liquid flow rate, gradient mixing, etc.; You can also use the optimized parameter to easily establish analytical methods; make data acquisition, data processing, qualitative analysis and quantitative analysis. Comply with GMP, GLP requirements, with user management authority control, data integrity, audit trail and other functions modules. 6 Computer system and printer 6.6.1 Brand host, LCD color display, genuine operating system software, matching the data processing system. 6.6.2 Laser printer, A4 black/ white. 7 HPLC system 7.1 High-pressure binary gradient pump-solvent delivery systemProgrammable design,reciprocating double plunger parallel pump with microprocessor intelligent control, with a highpressure-resistant value and small pulsation, reliable and easy to operate. The system also has a condition monitoringfunction as its microprocessor can achieve the real-time control of pump flow and detect the hydraulic pressure. 7.1.1 Flow range: 0.001 ~ 9.999mL / min (regulate the flow at the step size of 0.001mL / min) 7.1.2 Flow accuracy: RSD≤0.06% 7.1.3 Pressure resistance range: HPLC: Maximum 42Mpa, accurate to 0.1 Mpa (standard configuration) UHPLC: Maximum 60Mpa, accurate to 0.1 Mpa (can be selected according to configuration) 7.1.4 Pressure pulsation:≤0.1 Mpa 7.2 Manual injector: 7725i Manual Injection Valve 7.2.1 Injection mode: Full loop injection, partial loop injection 7.2.2 Injection volume: 1μL-20 μL 7.3 Column oven 7.3.1 Temperature range of the column oven: Room temperature ~ 80 ºC. 7.3.2 Temperature accuracy of the column oven: ± 0.1 ºC 7.3.3 Setting value error: within ± 1 ºC 7.4 UV detector 7.4.1 Wavelength range: 190 ~ 680nm. 7.4.2 Baseline noise: ≤ ± 0.25 × 10-5AU (empty pool) 7.4.3 Baseline drift: ≤0.4 × 10-4 AU (empty pool) 8 Basic technical parameters of ARCUS 5 Autosampler 8.1 Working temperature: 10~40ºC 8.2 Storage temperature: -25~+60ºC (no frost on the surface) 8.3 Sample loading: basic tray 2ml×54 bits;10ml×15bits;4ml×35bits; choose one type of pallet on each side, and any type of pallet can be placed on both sides, and the system automatically judges the pallet specifications 2ml×108bits(Factory standard configuration); Sample bottle height:H≤ 52mm(Including septa and cap); 8.4Loop volume: 1~120ul(Scalable factory standard configuration:50ul); 8.5Metering pump volume:200ul; 8.6 Injection mode:Full loop injection,partial loop injection,Sample lossless injection 8.7 Quantitative repeatability: Full loop injection:RSD6≤0.3% partial loop injection:RSD6≤0.5%(Injection volume≥10ul) Lossless injection:RSD6≤1.0%(Injection volume≥10ul) 8.8 Residue: ≤0.005% (perform a cleaning procedure) 8.9 Volume:450mm×300mm×330mm 8.10 Weight: 23kg (without packaging) FAQ
- What are the components of LC-MS?
LC-MS is an instrument that combines a liquid chromatograph with a mass spectrometer. Its composition includes two parts: a liquid chromatography system (HPLC, UPLC, etc.), a mass spectrometer (MS). Liquid phase LC310 is HPLC, divided into two configurations: isocratic and gradient.- How does LC-MS work?
The components of the sample separated by liquid chromatography enter the mass detector in turn, and the components are ionized in the ion source to produce ions with a certain charge and different mass numbers. Different ions have different motion behaviors in the electromagnetic field. A mass analyzer is used to separate the ions according to different mass-to-charge ratios (m/z) to obtain mass spectra arranged in the order of mass-to-charge ratios. Through the analysis and processing of the mass spectrum, the qualitative and quantitative results of the sample can be obtained.- What is a mass spectrometer?
The mass spectrometer is an instrument that uses the principle of electromagnetics to separate ions according to different mass-to-charge ratios and measure the intensity of various ions, thereby determining the molecular weight and structure of the measured substance.. - What are the common mass spectrometers for LC -MS?
Common mass spectrometers for LC-MS include quadrupole mass spectrometer (single quadrupole mass spectrometer, triple quadrupole mass spectrometer), ion trap mass spectrometer, time-of-flight mass spectrometer, Fourier transform cyclotron resonance mass spectrometer (FT- MS), electrostatic field orbitrap mass spectrometer (Orbitrap), sector magnetic field mass spectrometer, etc.. - What is the difference between a mass spectrometer coupled with liquid chromatography and a mass spectrometer coupled with gas chromatography?
Since liquid chromatography uses a large number of liquid mobile phases, it is necessary to vaporize the condensed liquid during mass spectrometry analysis,in order to meet the vacuum requirements of the mass spectrometer. Therefore, the mass spectrometer combined with liquid chromatography has a lot of advantages in the ion source interface and ionization mode. The big difference is mainly in the ion source. - What are the roles of each part of LC-MS??
LC:Realize the separation and concentration of organic matter, which can effectively remove interference. UV detectors can also be used to detect the concentration of separated organics, but the qualitative and quantitative capabilities of UV are far lower than mass spectrometers.MS:Provide molecular weight information and structure information of the substance to be tested, qualitative and quantitative functions.7. What are the parts of MS?The mass spectrometer mainly consists of ion source, mass analyzer, detector vacuum system and data processing system.8. What is the role of each part of MS?Ion source: vaporize and ionize liquid organic matter. And introduce the ion to be measured into the mass analyzerMass analyzer: Separate organic matter according to the mass-to-charge ratio.Detector: detects the positive and negative ions separated by the mass analyzer.Vacuum system: Provide a vacuum working environment for the instrument.9. Why does a mass spectrometer need a vacuum?The vacuum system provides sufficient mean free path for ions, provides collision-free ion orbits, reduces additional ion-molecule reactions, reduces background interference, eliminates discharge, and increases sensitivity.10. What is the resolution of LC-MS and what is the resolution of LC-MS2000??The ability to distinguish between two adjacent mass spectra peaks is expressed by the peak width at 50% of the peak height of an ion (half width for short), recorded as W1/2, the unit is atomic mass unit (u), LC-MS2000 is the unit Quality resolution (full width at half maximum ≤ 0.7amu).11. what is flow injection? When will flow injection be used? Does LC-MS2000 have a syringe pump?Flow injection is a way to use a syringe pump to directly feed the sample solution into the ion source without passing through the chromatographic column. It is used when entering relatively pure samples with less matrix interference, and can quickly screen the substance to be tested in a few seconds. Or use calibration reagents to calibrate the mass spectrometer or clean the ion source tubing. The LC-MS2000 comes with a syringe pump.12. What is selective ion monitoring? What is selective reactive ion monitoring? What is the full scan mode? What scanning modes does LC-MS2000 have?Selected ion monitoring: a method of detecting single or multiple ions of specific mass, referred to as SIM.Selected reactive ion monitoring: A method of detecting the product ions generated by the parent ion under a specific collision energy, referred to as SRM.Full scan mode: Full scan of masses in a certain quality range, referred to as Scan.Since the LC-MS2000 is a single quadrupole mass spectrometer, it has two scanning modes: SIM and Scan.13. What are the ion sources of LC-MS2000? Atmospheric pressure ionization(API)including Electrospray ionization(ESI)and Aatmospheric pressure chemical ionization(APCI),atmospheric pressure photospray ionization(APPI)etc.At present, the most used electrospray ionization source ESI (APPI and APCI are optional).14. What is atmospheric pressure chemical ionization APCI?It is chemical ionization under atmospheric pressure. With gas assistance, the solvent and sample flow through the sampling capillary, the sample and solvent in the capillary are heated and vaporized, and the sample aerosol is formed by spraying at the outlet of the capillary. There is a discharge needle downstream of the capillary, which uses corona discharge to make the gas Ionize with the solvent to generate reactive ions, and the reactive ions react with the sample to achieve sample ionization.- What is electrospray ionization ESI?
It is a soft ionization technology that uses a high electric field to charge the droplet flowing out of the capillary at the sampling end of the mass spectrometer. Under the action of nitrogen gas flow, the solvent of the droplet evaporates and the surface charge density continues to increase until the coulomb repulsion and the droplet surface are generated. When the tension reaches the limit, the droplet bursts into charged sub-droplets. This process is repeated to make the final droplet very small and foggy. At this time, the electric field on the surface of the droplet is very strong, so that the measured substance is ionized, and the single The charged or multi-charged ion enters the mass analyzer. ESI is divided into ESI positive ion mode and ESI negative ion mode.16. What is the difference between ESI and APCI in the scope of application?Ionization mechanism: Electrospray uses ion evaporation, while APCI ionization is the generation of [M+H]+ or [M-H]-ions due to the transfer of protons due to high-voltage discharge.Sample flow rate: APCI source can be from 0.2-2 ml/min; while the electrospray source allows a relatively small flow rate, generally 0.01-1 ml/min. Fracture degree: The probe of the APCI source is at a high temperature, and thermally unstable compounds will decompose. Scope of application: electrospray is beneficial for the analysis of large polar small molecules and biological macromolecules and other large molecular weight compounds (such as amines, quaternary ammonium salts, carbamates, proteins, peptides, oligonucleotides, etc.) , And APCI is more suitable for the analysis of less polar compounds (such as fatty acids, phthalic acid, urea, etc.). In practical applications, more than 90% of the analysis is done by ESI. Multi-charged: APCI source cannot generate a series of multi-charged ions. (There is a detailed comparison at the end of the article)17.What are the common mass analyzers for LC-MS?Ion trap mass analyzer, quadrupole mass analyzer, time-of-flight mass analyzer, Fourier transform mass analyzer, quadrupole mass analyzer, sector magnetic analyzer, etc.18.Performance characteristics of each mass analyzer? | Quadrupole | Ion trap | flight duration | Magnetic fan shape | FTMS | Quadrupole flight time |
Accuracy | 0.01% (100ppm) | 0.01% (100ppm) | 0.02to0.2% (200ppm) | <0.0005% (<5ppm) | <0.0005% (<5ppm) | 0.001%(10ppm) |
Resolution | 4000 | 4000 | 8000 | 30,000 | 100,000 | 10,000 |
m/z range | 4000 | 4000 | >300,000 | 10,000 | 10,000 | 10,000 |
Scan speed | second | second | Thousandths of a second | second | second | second |
Multistage mass spectrometry | Mass Spec 2 (Tripole) | Mass spectrum n | Mass spectrometry | Mass Spec 2 | Mass spectrum n | Mass Spec 2 |
Evaluation of Multistage Mass Spectrometry | High accuracy High resolution Low energy collision | High accuracy High resolution Low energy collision | Can't be widely used | Limited resolution High energy collision | Product ions have extremely high accuracy and resolution | Very high accuracy and resolution High sensitivity for low energy collisions |
19.What is MS/MS?The common types of MS/MS and tandem mass spectrometry are as follows:(1)Tandem quadrupole mass spectrometer,Compared with the single quadrupole mass spectrometer, it has better anti-matrix interference and qualitative ability, and has better quantitative reproducibility and detection sensitivity than ion trap mass spectrometry. The mass range and resolution are limited.(2)Quadrupole ion trap,Technically speaking, an auxiliary radio frequency is added to the quadrupole of the traditional QqQ, which can be used for selective excitation; or in terms of function, it provides a multi-stage cascade function for QqQ.(3)Quadrupole-time-of-flight mass spectrometry,QMS is used as a quality filter, and TOFMS is used as a quality analyzer.(4)Ion trap-time-of-flight mass spectrometry,The 3D ion trap is used as the mass selector and reactor, which combines the multi-stage mass spectrometry capability of the ion trap and the high resolution capability of time-of-flight mass spectrometry.(5)Linear ion trap-time-of-flight mass spectrometry,The linear ion trap is used as the mass selector and reactor, which combines the high sensitivity multi-stage cascade capability of the linear ion trap and the high resolution capability of time-of-flight mass spectrometry.(6)LTQ-FT LC/MS tandem mass spectrometer,Thermo Finnigan produces the LTQ-FT LC/MS tandem mass spectrometer, which realizes the perfect combination of the most advanced ion trap technology and Fourier transform ion cyclotron resonance technology, greatly improving the diversity of analysis. The analysis data obtained by the combined technology has the characteristics of ultra-high resolution, accurate mass and multi-stage mass spectrometry.20.How to achieve the vacuum of LC-MS2000?The vacuum chamber of LC-MS2000 is completed by a mechanical pump with a pumping speed of 30m3/h and two molecular pumps with a pumping speed of 350L/s.21.What is the ion source used by Skyray Instrument's LC-MS2000?At present, our instrument is equipped with electrospray ionization source ESI, atmospheric pressure chemical ionization source APCI is optional, and APPI is under development.22.What is the LC-MS2000 mass analyzer and what is the mass range? LC-MS2000 uses a single quadrupole mass analyzer with a mass range of 10-2000amu.23.The relationship between the mass range and the molecular weight of the test substance?The mass range is not equivalent to the molecular weight of the largest test substance. At present, the mass range of LC-MS2000 is 10-2000 amu, but some substances with a molecular weight greater than 2000 can also be tested, because some substances are multi-charged during ionization, such as polypeptides.24. Does LC-MS2000 have hardware protection function?LC-MS2000 has a protection function on the hardware. The molecular pump of the instrument is automatically started by the control of the instrument. When the first-level vacuum fails to meet the requirements, the molecular pump will not start. When the vacuum degree does not reach the standard, the instrument will power off the detector and the dynode.25.Can the mass spectrometer in LC-MS2000 be combined with liquid chromatographs from other manufacturers? Does it require other accessories??The LC-MS2000 mass spectrometer can be combined with any manufacturer's HPLC, UPLC and other systems without other accessories, but the LC-MS2000 software cannot control other companies' liquid chromatographs.26.Is the LC-MS2000 equipped with an autosampler? How many bits are there??The optional LC-MS2000 has an autosampler (ARCUS5). The autosampler can be configured with a maximum of 108 2ml sample bottle holes, which can be connected to any manufacturer's HPLC/IC system.27.The technical indicators of LC-MS2000 say that the unit mass resolution (half-maximum width ≤ 0.7amu) does not mean that LC-MS2000 can only reach this level?The technical indicators of LC-MS2000 say that the unit mass resolution (half-maximum width ≤ 0.7amu) is the test condition of the instrument, not the level that the instrument can reach. In the actual analysis, the resolution can be adjusted to a smaller value if the customer needs it.28.What brands of LC-MS are currently on the market, and how many are domestically produced?At present, the LC-MS manufacturers on the market include AB SCIEX, Thermo Fisher, PerkinElmer, Brook, Waters, Shimadzu, Agilent, Skyray Instruments, etc., and only Jiangsu Tianrui produces LC-MS in China. A Swiss Instrument Co., Ltd.29.What is the level of Skyray's LC-MS2000 compared with foreign manufacturers' instruments? What are the advantages?What is the level of Skyray's LC-MS2000 compared with foreign manufacturers' instruments? What are the advantages? Our LC-MS2000 has reached the same level as similar foreign products, and our instrument has a full Chinese interface, simple and clear operation, low cost of use and other advantages.30.What tuning fluid does Skyray Instrument's LC-MS2000 use? How often does the instrument need to be tuned? LC-MS2000 uses ESI tuning mix. For the instrument that is in normal use, we recommend that you perform manual tuning once a month for confirmation. For the instrument that is used for the first time or after a period of shutdown, we recommend that you perform tuning after the instrument is stable for 2 hours.31. Does LC-MS2000 have requirements for working environment?LC-MS2000 requires the ambient temperature to be used within the range of 18ºC~28ºC; the humidity is within the range of 20%~75%, the power supply voltage is within 220 V±5%, the frequency is 50±0.5 Hz, and the laboratory bench has no vibration, etc. .32.What are the consumables for LC-MS2000? Conventional consumables for LC-MS2000 include reagents, chromatographic columns, and nitrogen. At the same time, the detector of the mass spectrometer is also a consumable. Under normal use, the sensitivity of the instrument will decrease in 1-2 years, and the detector needs to be replaced.33.What are the requirements for the reagents used by LC-MS? The reagents used in LC-MS must be HPLC-grade or MS-grade, and the water must be ultra-pure water. The reagents used in sample pretreatment can be analytically pure as needed.34.What is the maintenance period of LC-MS2000?For instruments in normal use: clean the ion source flow path, check the air pressure every day, clean the ion source cavity every week, check the amount of mechanical pump oil, remove dust from the instrument every three months, replace the mechanical pump oil every six months, and perform other maintenance as needed .
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